analysis isosurface Search Results


thymus isosurface analysis  (Oxford Instruments)
99
Oxford Instruments thymus isosurface analysis
extl3/box -mutant zebrafish has defective thymopoiesis that is rescued by injection of EXTL3 wild-type RNA. (A) Extl3/box -mutant fish have shorter pectoral fins (arrows). (B) Immunofluorescence image of RAG2-GFP expression in 6–d postinfection (dpf) extl3/box Tg ( rag2:gfp ) larvae. Arrows indicate the thymus. (C and D) Representative images of <t>isosurface</t> 3D reconstruction (C) and total volume quantification (D) of thymic GFP signal from 6-dpf extl3/box Tg ( rag2:gfp ) larvae. (A–D) Shown are representative data of >50 sibling (sib) and 30 box animals from three independent experiments. (E) Rescue of thymic volume (left) and pectoral fin length (right) in 6-dpf extl3/box Tg ( rag2:gfp ) larvae injected with 100 ng EXTL3 wild-type RNA. Relative fin length indicates the ratio between lengths of fin to eye. n = 2 experiments. Error bars represent SEM. (F) Ext2/dak -mutant fish have shorter pectoral fins. (G and H) Representative images of isosurface 3D reconstruction (G) and total volume quantification (H) of thymic GFP signal from 6-dpf ext2/dak Tg ( rag2:gfp ) larvae. (F–H) Shown are representative images of >50 sibling, 30 box , and 27 dak animals from three independent experiments. Statistical significance was assessed using two-tail unpaired Student’s t test (D) and one-way ANOVA with Bonferroni post-analysis (E and H).
Thymus Isosurface Analysis, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/analysis+isosurface/Imaris/pmc05339678-155-3-11
Average 99 stars, based on 1 article reviews
thymus isosurface analysis - by Bioz Stars, 2026-09
99/100 stars
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isosurface function  (MetaMorph Inc)
90
MetaMorph Inc isosurface function
Loss of synMuv B proteins causes a delay in chromatin compaction in the intestinal lineage during development. (A) Schematic representing the number of intestinal cells and the approximate number of total cells in the stages scored throughout this study. EMS, the precursor cell to the E lineage. (B) Representative images of intestinal nuclei with open and closed extra-chromosomal arrays. In merged image, green is LacI::GFP and red is elt-2p::dTomato. (C) Intestinal nuclei were scored as open or closed based on array morphology (Yuzyuk et al., 2009) in worms raised at 20°C and 26°C at the 8E, 16E, comma and L1 stages. Asterisks represent significant difference between the wild-type population at that stage and temperature (P<0.01, two-way ANOVA). Error bars indicate s.e.m. (D) Representative masked images of intestinal cell nuclei with open or closed extra-chromosomal arrays using the <t>isosurface</t> function in Metamorph; green is LacI::GFP and red is elt-2p::dTomato. (E) The array volume to nuclear volume ratio was measured using the isosurface function in Metamorph at 8E, 16E and comma at 20°C and 26°C. Each dot represents a single intestinal cell and the line represents the median. Asterisk represents a significant difference between 20°C and 26°C (P<0.01, two-way ANOVA).
Isosurface Function, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/analysis+isosurface/isosurface+function+in/pmc06803374-441-4-4
Average 90 stars, based on 1 article reviews
isosurface function - by Bioz Stars, 2026-09
90/100 stars
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ivas 3.8.5 software package  (CAMECA Inc)
90
CAMECA Inc ivas 3.8.5 software package
Loss of synMuv B proteins causes a delay in chromatin compaction in the intestinal lineage during development. (A) Schematic representing the number of intestinal cells and the approximate number of total cells in the stages scored throughout this study. EMS, the precursor cell to the E lineage. (B) Representative images of intestinal nuclei with open and closed extra-chromosomal arrays. In merged image, green is LacI::GFP and red is elt-2p::dTomato. (C) Intestinal nuclei were scored as open or closed based on array morphology (Yuzyuk et al., 2009) in worms raised at 20°C and 26°C at the 8E, 16E, comma and L1 stages. Asterisks represent significant difference between the wild-type population at that stage and temperature (P<0.01, two-way ANOVA). Error bars indicate s.e.m. (D) Representative masked images of intestinal cell nuclei with open or closed extra-chromosomal arrays using the <t>isosurface</t> function in Metamorph; green is LacI::GFP and red is elt-2p::dTomato. (E) The array volume to nuclear volume ratio was measured using the isosurface function in Metamorph at 8E, 16E and comma at 20°C and 26°C. Each dot represents a single intestinal cell and the line represents the median. Asterisk represents a significant difference between 20°C and 26°C (P<0.01, two-way ANOVA).
Ivas 3.8.5 Software Package, supplied by CAMECA Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/analysis+isosurface/ivas+3+6+14+software/10__1017_slash_s1431927620024939-122-8-7
Average 90 stars, based on 1 article reviews
ivas 3.8.5 software package - by Bioz Stars, 2026-09
90/100 stars
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Image Search Results


extl3/box -mutant zebrafish has defective thymopoiesis that is rescued by injection of EXTL3 wild-type RNA. (A) Extl3/box -mutant fish have shorter pectoral fins (arrows). (B) Immunofluorescence image of RAG2-GFP expression in 6–d postinfection (dpf) extl3/box Tg ( rag2:gfp ) larvae. Arrows indicate the thymus. (C and D) Representative images of isosurface 3D reconstruction (C) and total volume quantification (D) of thymic GFP signal from 6-dpf extl3/box Tg ( rag2:gfp ) larvae. (A–D) Shown are representative data of >50 sibling (sib) and 30 box animals from three independent experiments. (E) Rescue of thymic volume (left) and pectoral fin length (right) in 6-dpf extl3/box Tg ( rag2:gfp ) larvae injected with 100 ng EXTL3 wild-type RNA. Relative fin length indicates the ratio between lengths of fin to eye. n = 2 experiments. Error bars represent SEM. (F) Ext2/dak -mutant fish have shorter pectoral fins. (G and H) Representative images of isosurface 3D reconstruction (G) and total volume quantification (H) of thymic GFP signal from 6-dpf ext2/dak Tg ( rag2:gfp ) larvae. (F–H) Shown are representative images of >50 sibling, 30 box , and 27 dak animals from three independent experiments. Statistical significance was assessed using two-tail unpaired Student’s t test (D) and one-way ANOVA with Bonferroni post-analysis (E and H).

Journal: The Journal of Experimental Medicine

Article Title: EXTL3 mutations cause skeletal dysplasia, immune deficiency, and developmental delay

doi: 10.1084/jem.20161525

Figure Lengend Snippet: extl3/box -mutant zebrafish has defective thymopoiesis that is rescued by injection of EXTL3 wild-type RNA. (A) Extl3/box -mutant fish have shorter pectoral fins (arrows). (B) Immunofluorescence image of RAG2-GFP expression in 6–d postinfection (dpf) extl3/box Tg ( rag2:gfp ) larvae. Arrows indicate the thymus. (C and D) Representative images of isosurface 3D reconstruction (C) and total volume quantification (D) of thymic GFP signal from 6-dpf extl3/box Tg ( rag2:gfp ) larvae. (A–D) Shown are representative data of >50 sibling (sib) and 30 box animals from three independent experiments. (E) Rescue of thymic volume (left) and pectoral fin length (right) in 6-dpf extl3/box Tg ( rag2:gfp ) larvae injected with 100 ng EXTL3 wild-type RNA. Relative fin length indicates the ratio between lengths of fin to eye. n = 2 experiments. Error bars represent SEM. (F) Ext2/dak -mutant fish have shorter pectoral fins. (G and H) Representative images of isosurface 3D reconstruction (G) and total volume quantification (H) of thymic GFP signal from 6-dpf ext2/dak Tg ( rag2:gfp ) larvae. (F–H) Shown are representative images of >50 sibling, 30 box , and 27 dak animals from three independent experiments. Statistical significance was assessed using two-tail unpaired Student’s t test (D) and one-way ANOVA with Bonferroni post-analysis (E and H).

Article Snippet: Image post-processing and thymus isosurface analysis and quantification were done with Imaris software (Bitplane).

Techniques: Mutagenesis, Injection, Immunofluorescence, Expressing

Loss of synMuv B proteins causes a delay in chromatin compaction in the intestinal lineage during development. (A) Schematic representing the number of intestinal cells and the approximate number of total cells in the stages scored throughout this study. EMS, the precursor cell to the E lineage. (B) Representative images of intestinal nuclei with open and closed extra-chromosomal arrays. In merged image, green is LacI::GFP and red is elt-2p::dTomato. (C) Intestinal nuclei were scored as open or closed based on array morphology (Yuzyuk et al., 2009) in worms raised at 20°C and 26°C at the 8E, 16E, comma and L1 stages. Asterisks represent significant difference between the wild-type population at that stage and temperature (P<0.01, two-way ANOVA). Error bars indicate s.e.m. (D) Representative masked images of intestinal cell nuclei with open or closed extra-chromosomal arrays using the isosurface function in Metamorph; green is LacI::GFP and red is elt-2p::dTomato. (E) The array volume to nuclear volume ratio was measured using the isosurface function in Metamorph at 8E, 16E and comma at 20°C and 26°C. Each dot represents a single intestinal cell and the line represents the median. Asterisk represents a significant difference between 20°C and 26°C (P<0.01, two-way ANOVA).

Journal: Development (Cambridge, England)

Article Title: C. elegans synMuv B proteins regulate spatial and temporal chromatin compaction during development

doi: 10.1242/dev.174383

Figure Lengend Snippet: Loss of synMuv B proteins causes a delay in chromatin compaction in the intestinal lineage during development. (A) Schematic representing the number of intestinal cells and the approximate number of total cells in the stages scored throughout this study. EMS, the precursor cell to the E lineage. (B) Representative images of intestinal nuclei with open and closed extra-chromosomal arrays. In merged image, green is LacI::GFP and red is elt-2p::dTomato. (C) Intestinal nuclei were scored as open or closed based on array morphology (Yuzyuk et al., 2009) in worms raised at 20°C and 26°C at the 8E, 16E, comma and L1 stages. Asterisks represent significant difference between the wild-type population at that stage and temperature (P<0.01, two-way ANOVA). Error bars indicate s.e.m. (D) Representative masked images of intestinal cell nuclei with open or closed extra-chromosomal arrays using the isosurface function in Metamorph; green is LacI::GFP and red is elt-2p::dTomato. (E) The array volume to nuclear volume ratio was measured using the isosurface function in Metamorph at 8E, 16E and comma at 20°C and 26°C. Each dot represents a single intestinal cell and the line represents the median. Asterisk represents a significant difference between 20°C and 26°C (P<0.01, two-way ANOVA).

Article Snippet: Analysis was performed using Metamorph isosurface function to determine the 3D distance between the two centroids as described by Yuzyuk et al. (2009) .

Techniques: